Study the effect of Topsin (Thiophanate-Methyl)fungicide on E. coli bacteria

Abstract

This study aimed to know the effect of three concentration of Topsin fungicide (0.05, 0.005,0.0005) mg/l on E. coli colony isolated from sediment of Euphrates river and find if possiblehalf lethal LC50 from pesticide to bacteria. The results appeared that different concentrationshave different effects on bacterial colonies growth which were average (137-232) colony/ml,(462-538) colony/ml and (605-657) colony/ml with percentage (17.41 -29.71)%, (60.16-65.44)% and (71.51-83.06)% to three concentration respectively. The result pointed to thevalue of LC50 was 0.0099 mg/l. Also test tubes that have pesticide showed growth, whichpointed to bacteria adaption for these concentration. The results pointed to a significantdifferentiation between growth average in probability at level (0.05) and reverse correlationcoefficient between concentration and average of growth.