Isolation, Characterization and Estimation of Ellagic Acid activity against Porphyromonas gingivalis Isolated from adult Periodontitis Patients in Kerbala City.

Abstract

Periodontitis is a chronic bacterial infection affects the gingiva, periodontium connectivetissues and alveolar bone, results in alveolar bone resorption, ultimately, partially orcompletely tooth loss, indeed, it may causes various serious systemic complications likeDiabetes Mellitus, Cardiovascular disorders, Rheumatoid Arthritis, Preeclampsia with lowbirth weight and Orodigestive cancer mortality. Porphyromonas gingivalis is considered themain, foremost and strongest periodontal pathogen. Isolation, characterization and monoplexPCR of 16S rRNA and multiplex PCR of fimA geneotyping are the golden standard assays fordetection of P.gingivalis in adult periodontitis patients. In the present study,Pomegranate peelEthanol extract partially purified ellagic acid has an obvious effectiveness against oralpathogens (P. gingivalis and S. mutans) in both growth inhibition zone on solid medium andPercentage of growth reduction in a liquid medium in various concentrations with commonMIC 16mg/ml.