25- BACTERIOLOGICAL ISOLATION AND EVALUATION OF THE ANTIMICROBIAL RESISTANCE OF ENTEROBACTERIAL ISOLATES FROM GALL BLADDER OF SLAUGHTERED CATTLE

Abstract

The increasing prevalence of antimicrobial resistance in meat-producinganimals, especially ruminants, represents a major problem for human and animal andalso could increase the patient's morbidity and mortality. The gallbladder may be a sitof persistence and a source for fecal shedding of certain enteric food-borne pathogenresistant to many antimicrobial agents. In the current study 80 samples (32 bile, 48epithelium) were examined to isolate the enteric pathogen;AII samples were culturedon primary and selective. The frequency of isolation of microorganisms was (60% )inthe epithelium and (40% ) in the bile. The major pathogen isolated were (68.57%)Proteus spp. (21.25%)E-coli ,(7.5)Citrobacter ,(1.25) Psudomnas.and(1.25)Klebsiella.The antibiotic resistance was determined by Kirby-bauer discdiffusion method using 10 of routine and practical antibiotics. In antimicrobial testingfrom both bile and gallbladder epithelium showed sensitivity to the followingantimicrobial :amikacin, cefoxitin, chloramphenicol, gentamycin, kanamycin andciprofloxacin.In conclusion , the current study provide helpful insights into the prevalence of foodsource pathogens. High level of antibiotic resistance in proteus spp and Ecoli thatcould transmit to humans through meat and meat products need for monitoringsystem on the incidence and antimicrobial susceptibility of enteric pathogens in meatanimals in slaughterhouses.