DETERMINATION OF OPTIMAL CONDITIONS FOR PROTEASE PRODUCTION FROM LOCALLY ENVIROMENTAL BACTERIA Aeromonas hydrophila ISOLATED FROM SURFACE WATERS FOR TIGRES RIVER IN BAGHDAD

Abstract

Thirty local isolates locally of Aeromonas hydrophila were isolated from (80) water samples and tested for protease enzyme production by using skim milk agar . All isolates were able to develop clear zone around the colonis and select of Aeromonas hydrophila A30 as the most efficient producer.Optimization studies revealed that tryptone broth was the best at initial (pH 7.5) ,at 35° C for 24 hrs .