EXPERMENTAL STUDY TO COMPARE BETWEEN THE EFFECT OF ALCOHOLIC AND AQUEOUS EXTRACT OF CLOVE ON THE GROWTH OF STAPHYLOCOCCUS AUREUS ISOLATED FROM MASTITIS IN COWS INVITRO AND INVIVO

Abstract

This study was conducted to investigate the role of alcoholic and watery extract of clove onthe invitro Inhibition if staphylococcus aureus , which were Isolated from acute bovine mastitis and thetherapeutic role of these extract in mammary gland of mice which were used as animal model in experimentalinfection design. Inorder to study the pathogensis of these Isolates and their role on acut mastitis studyincluded the therapeutic effect of watery extract of clove in the Mammary gland infection compared with thepositive group (mice injected withs staph . aureus without treatment) .The watery extract of clove dissolved inwater (300) mg /ml showed highly Inhibition effect on stuph . aureus growth in vitro so its gave ( 53.3) mminhibition zone while the alcoholic extract which dissolved in water showed the lowest effect with (35 ) mmzone by the same concentration at (300)mg/ml . study inclued also the therapeutic effect of daily orallytreatment dose 100mg /ml of watery extract of clove in the patgogensis of staph.aureus in these treatmentperiod continous (30) day after 48hr of injection 125cfu/ml in mice in addition to use positive control group(mice injected with staph . aureus with out any treat ment) , clinical signs were shown side effect of cloveextract after 15 day of treatment . histological study showed the role of clove extract it’s the same ashistopathologcal changes in compared with the negative control and the mammary gland of these animalshowed negative growth for staph.aureus .