Relationship of Growth Hormone Gene with Some physiological Characteristics of Common carp Cprinus carpio.L

Abstract

This study was carried out at Al-Radhwaniyah Fish Reservoir (Baghdad) for the period from 16/9/2016 to 16/2/2017 to investigate the polymorphism of GH Gene and Relationship with glucose, cholesterol, AST, ALT, HDL and LDL in common carp. Single nucleotide polymorphism (SNPs) in GH gene was analyzed by direct sequencing. Results showed that there was a mutation in the site of G1217T, this SNP was significantly (p<0.05) associated with the concentration of glucose and cholesterol in the blood. Heterozygote genotype (GT) recorded glucose concentration of 79.90 mg / 100ml while wild genotype (GG) 67.27 mg/100ml. Cholesterol reached 147.84 mg / 100 ml in GT genotype and 129.16 mg/100ml in GG genotype. The Results indicated was no significant associated with AST, ALT, HDL and LDL characteristics. The conclusion of this study, the polymorphism of GH gene is possible to adopt as a mark to predict the performance and selection of fish to be parents for future generations.