New Spectrophotometric Method for Determination of Ceftazidime in Pure Form and Pharmaceutical Dosages

Abstract

A new simple, rapid, inexpensive and nontoxic spectrophotometric method for the determination of Ceftazidime in the pure and local market formulation was proposed. The method is based on the coupling reaction between the diazotized drug and 4-tert-butylphenol (4-TBP) to produce a colored compound with a (λmax 500 nm). The optimal conditions for the factors affecting the formation of this compound were studied. Beer's law was obeyed and a linear calibration curve was obtained over the range of (1–10 μg/mL) with a correlation coefficient (r = 0.9890), molar absorptivity (ε = 6.627  103 L/mol.cm), the limit of detection 0.314 μg/mL, and the limit of quantification LOQ 1.047 μg/mL. the accuracy and precision of the method were checked and the method was applied successfully for the determination of Ceftazidime in commercial preparation and the values of recovery percent and RSD% ranged between (93.11 - 102.20%) and (0.48 - 9.54%) respectively.