STUDYING THE EFFECT OF GINGER ROOTS EXTRACTS ON lVIICROORGANISiVIS

Abstract

Many extracts were achieved from ginger roots included water extract, alcoholic extract, water extract with ccllulascs produced by .sPC!•gi!!l!S, :md water extract by reflux 'extraction method. Their effects agrtin't some microorganisms such as Salmonella typltinutriunt, E. coli, Bacillus cereus, Stapliyllococcus aureus, Pseudomonas aeruginosa and Candida albicans were studied. Antimicrobial activity was estimated by Disc diffusion assay. Results were showed that the water extraction of ginger' had an antimicrobial effects against E.coli and Stuphylococus aureus at concentration 100 mglml. The average diameter for inhibition zones were 7.5 and 6.0 mm. respectively, also it was shown an antimicrobial effects against Puterugiuosa and Candida albicuns at concentrations 25,50 and 100 rug Iml . The highest effect was found against C. albicuns, which the average diameter for inhibition zone was 9 mm. On the other hand S. typhimurinm and B. cereus were not effected by this extract. The alcoholic extract had the highest antimicrobial effect against Staph. anrcus with average diameter 10.5mm, beside its effect against Piaerugins, C. albicans, S. typhimurlum and E. coli, but there was no effect against Bcereus. Using cellulases for rapture the ceI! walls cause the extraction of more antimicrobial compounds and the reflex condense extract had an antimicrobial activity against all the microorganisms tested in this study except E. coli. In conclusion, using of cellulases in extraction supports increasing the extracted compound especially antimicrobial compounds from plants because of their cellulolytic effect on plant cells, so we can use ginger extraction as an antimicrobial agent to keep food from spoilage and deterioration.