دراسة في بعض التغيرات الهرمونية وعلاقتها بالمعايير الفسلجية للسائل المنوي لمرضى عدم الخصوبة

Abstract

The aim of This study was investigating the hormonal levels relationship between hormone and physiological parameters of sperms in some infertile patients in Kerbala. The levels of Luteinizing hormone (LH), Prolactine hormone (PRL), Testosterone (T) in blood serum, and microscopic examination of seminal fluid have been measured. Part of this work was done in the laboratories of Kerbala where the samples was collected, while the other part was done in the laboratories of Biology department, college of Education Kerbala university .Blood samples were collected from infertile men .There were 49 men in different cases of infertility including. 26 cases with Asthenospermia , 12 cases with Oligospermia,and 11 cases with azoospermia. We also have taken 11 fertile men (they have children) as a control in the study to correlate a change in the data studying.the sera were separated ,Then the levels hormones were measured using Enzyme linked Immunosorbrnt assay (ELISA) of hormonal analysis, the results could be summarized as follows:Measured of levels hormones was appeared there was significant increase (P<0.05) in the levels of luitinizing hormone in Oligospermia patients, while there were no significant differences in the levels of hormone in the Asthenospermia and Azoospermia. There was significant increase (P<0.05) in the levels of Prolaction hormone in Oligospermia and Azoospermia patients, while there were no significant differences in the levels of the hormone in Asthenospermia also there was significant decrease (P<0.05) in the levels of testosterone in Asthenospermia and Azoospermia while there were no significant decrease in the levels of that hormone in Oligospermia. While the relationships between hormones and other parameters could be summarized as follows:There was no relationship between the levels of LH hormone and concentration of sperms and grade activity in Asthenospermia while there was significant relationship (P<0.05) between the levels of LH hormone and percent of sperms motility and there was no significant relationship (P>0.05) between levels of LH hormone and percent of abnormal morphology of sperm, while there was significant relationship between the levels of LH hormone and the mean of white blood cells in all taken infertile men.While Oligospermia there was significant relationship between the levels of LH hormone and concentration of sperms and the degree of grade activity, while there was no significant relationship between the levels of LH hormone and the percent of sperms motility, while there was no significant relationship between the levels of hormone and the percent of abnormal sperms.There was no significant relationship between the levels of prolactine hormone and concentration of sperms in Asthenospermia and Oligospermia, while there was significant relationship between the levels of of hormone and the percent of sperms motility in Asthenospermia and Oligospermia and there was no significant relationship between the levels of hormone and the degree of grade activity in Asthenospermia and Oligospermia, while the relationship was no significant between the levels of hormone and percent of abnormal sperms in Asthenosperms while there was no significant relationship between the levels of hormone and the number of white blood cells in Asthenospermia and Azoospermia, while Oligospermia there was no relationship. While there was no relationship between the levels of testosterone and concentration of sperms in Asthenospermia, while there was significant relationship in Oligospermia , there was significant relationship between the levels of hormone and the percent of motility sperms and the degree of agrsde activity in Asthenospermia and Oligospermia, while there was no significant relationship between the levels of hormone and percent of abnormal sperms, there was significant relationship in Oligospermia, while there was significant relationship between the levels of hormone and the mean of white blood cells in Asthenospermia and Oligospermia, while there was no significant relationship between it and the levels of hormone in Azoospermia.