Isolate the enzyme Nuclease of local isolation Staphylococcus spp. EH69

Abstract

One hundred thirty eight samples were collected from human skin. Thirty two isolates (23.1%) were found to be Staphylococcus. The isolates were subjected to primary and secondary screening for nuclease production. The isolate Staphylococcus EH69 was found to be an efficient nuclease producer in submerged culture. The optimum environmental conditions for enzyme production were found to be using Brain Heart Infusion supplement with glucose and sodium bicarbonate medium at optimum pH 8 and inoculum size 3% at 35Co.