Identifcation of some local wheat varieties by high performance liquid chromatography technique

Abstract

Gliadin protein of three soft wheat cultivars namely Abu-Ghraib, IPA 99 and AL-Littefia was extracted and separation by high performance liquid chromatography HPLC. Result showed that there were significant differences between the gliadin percentage ratio with the order of AL-Littefia 34.02% , IPA99 30.20% and Abu-Ghraib 31.00% . Also the result of HPLC analysis revealed that the gliadin of the test cultivars has 3 distinguish patterns of separation. Thus it appears that HPLC provides efficient tool for identification of wheat cultivars. Abu-Ghraib gliadin showed 6 peaks while IPA99 and AL-Littefia showed 15 and10 peaks respectively.