Effect of Lawsonia inermis extract on the pathological changes of skin infection by Streptococcus pyogens in lab. Mice

Abstract

Effect of Lawsonia inermis extract on the pathological changes of skin infection by Streptococcus pyogens in lab. MiceB. M. M. Al-Mehna E. A. H. Kadhum Coll. of Vet. Med. Univ of Al-Qadisiya AbstractIn this study, the antibacterial effects of aqueous and alcoholic extracts of Lawsonia inermis leaves against Streptococcus pyogenes were investigated in vitro by using agar well diffusion method and in vivo using laboratory mice by treating it with prepared ointment from these extracts & compared the effects wih Gentamicin . Results showed that both extracts demonstrated antimicrobial activity against the tested organism but the efficiency of the extracts was significantly affected by the solvent used in the extraction as well as the concentration of extract. Alcoholic extracts had the highest antibacterial activity it exhibited an inhibition zone 18.2-28.2 mm in comparison with 26.2 mm for gentamicin .A prepared ointment from alcoholic extract in a concentration 5% was tested for treatment of the experimentally infected skin of mice by S. pyogenes using scratching . The recovery period was 11 days in compared with 10 days for gentamicin. Biopsy was taken from the infected area to detect the pathological effects occurred by tested bacteria ,also biopsy was again taken after the treatment with the prepared ointment to evaluate the anti-inflammatory ,antibacterial and wound healing activity of Lawsonia inermis. Histopathological study showed after 3days from skin infection with Streptococcus pyogens hypremia, swelling, congestion and pus formation also abscessation While in treated animals with henna extracts gross skin lesions disappeare after 11 days from infection and on day 10 showed increased well organized bands of collagen, more fibroblasts and few inflammatory cells when compared with the controls which showed inflammatory cells, scanty collagen fibres and fibroblasts.