Isolation and Screening of Glutamic Acid- Producing Microorganisms

Abstract

More than 1000 microorganism species isolated from soil were exposed to primary and secondary screening by bioassay, paper chroatography (PC) and high performance liquid chromatography (HPLC) to find glutamic acid- producing species and determine the best isolated in production of glutamic acid. Leuconostoc mesenteroides P4, isolate in the present study was used as test organism for bioassay of glutamic acid. Results of primary screening revealed that 140 isolates had the ability to produce glutamic acid. Of these, 61 isolates showed distinctive spots of glutamic acid in PC with special superiority of 14 isolates. It was found that 54.1% of isolates tested by PC belonged to coryneform bacteria. Secondary screening showed that one isolate produced 5.787 mg/ml of glutamic acid represented 67.45% of total amino acids. It was found, after identification that this isolate belonged to Corynebacterium glutamicum.