Influence of Osmotic Pressure & Incubation & some Material on the Production of " Scleroglucan " from Sclerotium rolfsii.

Abstract

The production of Polysaccharine (Scleroglucan) has been completed by one of the strain of fungus S. rolfsii and by using one of the cultivate media under an incubation cultivate state, when the salt ( NaCl ) added to this media led to high increase the Sclerogan production as a result for increasing the osmotaic pressure which is raised from adding the salt with incubation concentrations. As well as the production effect of Sclerogan was on the difference incubation terms and it's high production was on the five days which reached ( 16gm / 1). So as adding some materials such as Thiamine hydrochloride, ascorbic,ZnSO4, 7H2O Olive oil and Cellibios did not lead to any increase of Sclerogan production by the fungus S. rolfsii.