ADENOSINE 3' , 5' – CYCLIC MONOPHOSPHATE : EXT RACION AND PURIFICATION FROM URINE OF β – THALASSAEMIA PATIENT

Abstract

Abstract Cyclic adenosine 3',5'-monophosphate has been extracted and purified using column chromatography ( Dowex 50-w column of 1.5x 5 cm dimentions ). Levels of the purified adenosine cyclic monophosphate peak was determined and it was seen to be elevated in urine of thalassaemic patients in comparison with that of healthy controls objects. The purified peak was identified using UV analysis