Using Of Culture Media Added to Woman Blood serum to Activate Epididymal Spermatozoa in Vasectomized Rabbit

Abstract

An experiment investigated the effect of three different periods ( three, five and nine weeks on the viability of the epididymal spermatozoa of a vasectomized rabbit. Three different cultural media viz: 5% dextrose ,sodium citrate and tris with addition of 20% and 40% of women blood serum to activate the epididymal spermatozoa were also studied. The results revealed that a significant decrease in epididymal spermatozoal viability was noticed after five weeks of vasectomy before spermatozoal activation as compared with the result of after activation. There were a significant increase in spermatozoa viability when dextrose and sodium citrate media were used with a significant decrease in sperm concentration. The tris medium led to decrease in the viability of epididymal spermatozoa in all the three periods