Studying of antibacterial effect for leaves extract of Callistemon viminalis in vitro and vivo (urinary system) for rabbits

Abstract

This study was designed to evaluate the effects the watery and alcoholic extracts from leaves of Callistemon viminalis in vitro to ten of pathogenic bacteria and study the alcoholic extract toward Streptococcus pneumonia on urinary system in vivo. The results showed the watery and alcoholic extracts from leaves of Callistemon viminalis have antibacterial activity against both Gram positive (Staphylococcus aureous – Streptococcus pneumonia – Staphylococcus epidermidis ) And Gram negative bacteria (Klebsilla pneumonia – Klebsilla oxytaci –Proteus vulgaricus - Escherichia coli) however the watery extract of Callistemon viminalis were more potent than alcoholic extract against pathogenic bacteria (80%) . According to results , Streptococcus pneumonia pathogen that more sensitive to ward Callistemon viminalis alcoholic extract was choosing to injected intraperitonialy as experimental infection in laboratory animals (vivo) which cause morphological and histopathological degenerative lesion of kidney cortex and medella tissue in addittion to change of renal profile test that include blood urea nitrogen, creatinine, creatinine kinase, uric acid, inaddition to calcium. but after alcoholic extract of Callistemon viminalis injected in these labratory animals cause significant improvment (p≤0.01) in the value of blood urea nitrogen, creatinine, creatinine kinase and uric acid. Calcium concentration has small improvement but don't reach significant degree, histopathological studies confirm these results which include regeneration of degenerative lesion for medulla and kidney cortex with convoluted tubules tissue