In vetro effect of Gibberellin and cultivar on induction and sprouting microtuberization potatoes cv. Diamant, Desiree, and Marfona

Abstract

This stady was conduted during the period 2009 / 2008 in the tissue culture lab. Biotechnology Department- Technical College / Musaib. Sprouts of Diamant, Desiree and Marfona potato tubers ( 0.3 – 0.5 mm) were transferred on the propagation medium. Explants were sterilized with sodium hypochlorite at 5% for 15 min., cultures were incubated at 25º C ± 2 and 16 hours photoperiod at light intensity 1000 lux for 35 days. The (1 – 2 cm.) basal parts of the producing cuttings were grown on microtuberization MS media containing 8% sucrose plus 2.0 g / l charcoal. The first study contained treating the microtubers with Capitan at 2g / 1kg tubers to study the effect of cultivar and size on the percentage of the damaged tubers, sprouted tubres, number of developed buds/ tuber and vegetative buds / tuber, before and after the storage. The second trial was about the effect of cultivar and Gibberellin ( 0.0, 3.0, 4.0, 5.0 and 6.0 mg / l ) in the characters studied. The interaction between cultivar and size had a significant influence. Hightest results were obtained from Desiree with the larger size S1 regards bud number / tuber, number of grown buds, ( 6.97, 5.31 ) respectively, wheras, Diamant with S1 resulted in less damaged tubers and higher sprouting ( 4.05%, 99.29% ) respectively. Interaction of Desiree with GA3 at 5.0 mg / l had significant effect on number of developed buds ( 5.31), while Diamant with 5 mg / l Gibberellin gave higher sprouting ( 89.14% ) on the other hand there was a decrease in dry metter for all cultivars.