عزل وتشخيص البكتريا المقاومة للفينول من تربة الحدائق

Abstract

Thirty bacterial isolates capable of phenol resistance were isolated from garden soil in Hilla city. By standard bacteriological methods, these bacteria were belong to genera Staphylococcus aureus, Bacillus subtilis and Proteus sp.. Seven of these strains were found resistance to 10 mM of phenol. Using of acridin orang determined that phenol resistant genes are located on plasmid. Plasmid DNA isolated from the uncured strains was transferred into the cured competent recipient cells. Stable transfer of phenol resistance plasmid gene(s) was observed.