تأثير طرائق الاستخلاص المتعددة في كميةمتعدد بيتا هيدروكسي بيوتاريت (PHB)المستخلص من بكتريا Burkholderia cepacia وتأثيرها في وزنه الجزيئي

Abstract

PHB was extracted from Burkholderia cepacia locally isolated by five different methods iculding sodium hypochlorite(with different concentration ) ,by sonication ,glass beads –SDS- sodium hypochlorite,ethyl alcohol –chloroform and sodium hydroxide .The glass beads –SDS- sodium hypochlorite method at 50and pH 11 was more efficient than other methods in PHB extraction 57%PHB from cell dry weight than sodium hypochlorite method 51%PHB.The effect of extraction methods on molecular weight was studied,it observed The molecular weight of the PHB was 4.7×105 dalton when ethanol –chloroform was used while it was 3.8×105,3.8×105 ,2.4 × 105 dalton when sodium hypochloriteand , sonication , glass beads –SDS- sodium hypochlorite , sodium hydroxide respectively .