Immunohistochemical study of CD34 in tooth eruption by using amniotic stem cells

Abstract

Background: Tooth eruption is a more general process, however, which includes certain posteruptive toothmovements. There are two fundamental requirements for both tooth eruption to occur:(1) Require soft tissue, intervening between tooth structure and alveolar bone, which plays an important role inregulating the remodeling of adjacent tissues.(2) Require bone turnover that is temporally and spatially regulated to facilitate specific translocations of teeththrough alveolar boneThese amniotic stem cells are multipotent and able to differentiate into various tissues, which may be useful forhuman application and recently it used in many medical branches. CD34 is an endothelial marker that is extensivelyused in immunohistochemistry and most vascular endothelial cells. Expression of the stem cell antigen CD34 is adefining hallmark of hemopoietic stem cells and progenitors. This study aimed to study the expression of CD34 bydental cells involved in tooth eruption after administration of amniotic stem cellMaterials and Methods: forty eight albino Swiss mice of one day old age injected with isolated amniotic stem cells inthe anterior region of maxilla (incisors area) other 16 mice injected with saline represents control. Sacrifice 4 mice foreach period (4, 7, 10, and 13) day old age. The result were studied histologically and immunohistochemistry.Results: Immunohistochemical result revealed positive expression of CD34 in pulp (Vascular, Paravascular),Mesenchymal cell and in the Dental sac of different groups. Coincidence test of expression marker CD34 in variousstudied group shows that Chorion application affected on CD34 expression in pulp while Amniotic fluid affected ondental sac.Conclusion Immunohistochemical study of expression marker CD34 in various studied groups show that chorionapplication affected on CD34 in pulp .While amniotic fluid affected on dental follicle