استخلاص وتنقية البروتين المرتبط بالفايبرونكتين من بكتريا المكورات العنقودية الذهبية

Abstract

The extraction and characterization of fibronectin binding protein from cellular wall of Staphylococcus aureus, was carried out 12 out of 40 isolates bacterial specimens had been selected which had been get from medical city laboratories , public health laboratory and biotechnology department of college of science / Baghdad university . All diagnostic confirm test had been done in order to be sure of its purity and ability on production of fibronectin binding protein .Extraction of protein included, bacterial suspension centrifugation by cooling centrifuge followed by bacterial cells destruction by sonicater device , also protein extract had been concentrated by ammonium sulphate , followed by dialysis operation and ultra filtration in order to get ride high percent of salts and reasted which are present in solvent . protein Purification results were revealed that molecular weight by gel filtration chromatography method , on the other hand electrophoresis method on polyacrylamide gel at present of denatured materials for protein SDS and mercaptoethanol molecular weight was (59000 ) and (58000 ) dalton approximately , the protein was afibronectin binding protein via the compared of protein with standerd proteins .