And prepare Msalh using anti yolk eggs (ToMV) diagnosis and virus purification Mozaik tomato Almmna chicken.

Abstract

Tomato mosaic virus (ToMV) was diagnosed by using indicator plants.ToMV was purified by differential centrifugation, after filtrate by agarose gelfiltration, from tobacco leaves its purity and concentration were 1.3 and 1.3mg/gm. plant juice respectively , and it was effective in inducing infection onleaves of wild tobacco. The isoelectric point of the virus was 4.4 pH. Theantiserum of ToMV was prepared by using immunized chicken eggs, and wastested by agar double diffusion and slide agglutination tests.