التصنيف العددي بالتحليل العنقودي للبكتريا غير المخمرة المعزولة سريريا من إحدى مستشفيات بغداد

Abstract

Thirty eight isolates belonging to the non-fermenterbacilli were isolated and typed depending on the tests included on the API 20Ekit and its complimentary tests, the isolates were given 9- digital bionumber known as Apibiopattern-2, by the use of Word Wide Web and through the compact disc named apiwebTm V 1.1.0 produced in January 2006 an access to the database that belongs to the company producing the kit were done, Which made the diagnosis of these isolates possible, depending on these biopattern plus the results reprents its physiological, serotyping and itsantibiogram, the isolates were classified numerically by cluster analysis by use of Jaccard's coefficient and a dendrogram were drawn showing the percentage of similarities between the isolates. Four main clusters were produced; each single cluster included most isolates belonging to single species or group in different similarity levels.Two isolates belonging to the species Pseudomonas aeruginosa, and two others belonging to the group.Pseudomonas fluorescens/ putida recoded the highest similarity level (Sj ≥82.4%). The highest similarity level between two isolates belonging to different species were recorded, one isolate belonging to the species Ochrobactrum anthropiand the other belonging to the Non-Fermenter spp. showing (Sj≥75.0%).The bacterial total soluble protein profile for (5) different isolates under study were investigated by using SDSgel electrophoresis,60% of the isolates seemed to harbour protein bands of approximate molecular weight73, 104, 167 kD.