تنشيط النطف البربخية في الفئران باستخدام مصل دم النساء الفاعل المضاف الى اوساط زرعية مختلفة

Abstract

The objective of this research work was to induce in vitro activation of Epididymal Spermatozoa in adult fertile male Mice (Mus musculus) by using different culture media to compare between them by studying:1.The effect of Fresh Blood Serum of Women with different concentration (20%, 50%) on Sperm Parameters in in vitro activation.2.The effect of two different culture media on in vitro activation of Epididymal Sperm & determine the best one between them.The adult fertile male Mice (Mus musculus) used in this research. Modified Earle`s Medium (Baghdad Culture Medium) (BCM) & Dextrose Medium (5%) supplement with either 20% or 50% Women Mid – Cycle Serum was used as culture medium. Simple Layer in vitro sperm activation Method submitted in this research.The use of 20% fresh women serum in (BCM) gave significantly (P<0.05) highest sperm activation compared with 50% fresh women serum in Dextrose (5%). Sperm motility and grade activity significantly increased (P<0.05) in (BCM) compared with Dextrose (5%) medium.It concluded from the results of this study that the use of Simple Layer in vitro sperm activation method with (BCM) supplemented with 20% fresh women serum resulted in significant improvement in Epididymal sperm motility and grade activity