Diagnosis of Brucella abortus infections in patients suffer from PUO InBasrah governorate

Abstract

A total number (130) of patients whom suffering from pyrexia unknown origin blood samples were collected, 20 sera sample from healthy individuals (blood donors) were taken and used as control group. Out of the total number of samples 11(8.4%) were showed growing isolate of Brucella abortus on Castaneda Di-phasic medium which were diagnosed by biochemical tests. Enzyme Linked Immune Sorbent Assay (ELISA) was done for screening IgM and IgG, the results were revealed from 50 samples, 25(50%) positive for IgM in acute infections and 7(14%) positive for IgG in chronic infections. All 20 control samples were negative for the previous tests.