Evaluation the effect of hyaluronic acid on bone healing process in rabbits (Immunohistochemical study for TGF-β)

Abstract

Background: Bone augmentation techniques are commonly employed in medical fields. This biomaterial system mustbe readily available, easily applicable by minimally-invasive technique and able to release an osteoinductivegrowth factor. Such a system will be able to engineer new bone formation locally at the site of injection. Hyaluronicacid has osteogenic potential that can be exploited not only for repairing bone defects but also for providingtransplantable bone for the reconstruction of a variety of bone defects. The aims of this study were to evaluate theeffects of Hyaluronic acid gel on bone healing by immunohistochemical estimation of transforming growth factor -beta 3 in experimental and control groups.Materials and methods: Thirty two New Zealand male rabbits were used in this study .Two Intra bony holes were madefor each rabbits on the right and left buccal side of the upper diastema. The right hole was filled with Hyaluronic acidgel (experimental one), while the left hole was left for normal healing (control one). The rabbits were randomlydivided and sacrificed at 1, 2, 3 and 6 weeks post operatively. Immunohistochemical test for the expression of TGF-β3were performed on bone specimens of both control and experimental groups at all healing interval.Results: Immunohistochemical examination of this study revealed that the hyaluronic acid treatment increased thepositive expression of TGF-β3by osteoblasts, osteocytes and bone marrow stromal cells especially in 1 and 2 weeksintervals than that observed in control one.Conclusions: The present study illustrated that the Hyaluronic acid was osteoconductive material that enhanceosteogenesis and accelerated the bone healing process