Protein pattern changes and determination salt tolerant proteins of okra (Abelmoschus esculentus Moench.) plants irrigated with salty water and treated with potassium nitrate and Tocopherol

Abstract

An experiment was conducted in a private orchard at Shat-Al- Arab District, Basrah Governorate during the summer seasons of 2011and 2012 to study the effect of irrigation water quality and spraying with potassium nitrate and Tocopherol on protein pattern changes of okra plants.Treatments included three sources of water i.e. tab water, tab water + river water and river water alone or with 30 m. mol. potassium nitrate and150mg. L.-1Tocopherol. Result showed that the total number of protein bands/lane did not change under the low salt concentration but was dramatically reduced by the high salt treatment. The sum of optical densities (O. D.) of protein bands was inhibited by both levels of irrigation water, but was induced with the addition of KNO3 and Tocopherol with tab water+ river and with river water. different polypeptides of molecular weights were completely disappeared under river water irrigation. These bands reappeared in response to the addition of KNO3 and Tocopherol. Moreover, the optical density of every individual protein band was induced by Tocopherol under the low salinity. Results indicate synergistic interaction between salinity stress and KNO3 and Tocopherol for the sake of salt tolerance in okra plants.