دراسة التغييرات النسيجية والكيموحيويه المستحثه بفعل السم الفطري زيرالينون في الفئران البيض

Abstract

Study the Histological and Biochemical Changes Induced by Mycotoxin Zearalenone in White MiceDr.Shatha Ali Shafiq ;Mushtaq Talib KareemDepartment of Biology , College of Science – Mustansiriyah universitySummaryThe present study was to evaluate the histological and biochemical toxic effects of mycotoxin Zearalenone on liver and kidney tissues of males white mice that extracted from fungal isolate Fusarium graminearum produced Zearalenone at concentration (2 mg / kg body weight) given single and repeated doses of toxin via oral administration. Mice were divided into three groups (five mice for each group), taking into consideration body weight, liver and kidney weights. T1: receiving the toxin once and sacrificed after two days. T2: given the toxin twice for one week. T3: given the toxin twice a week for two weeks. Each treated group has its corresponding control which received 1% Dimethyl Sulphoxide (DMSO).The results showed the histological changes in liver tissue in T2 and T3 groups which represented by congestion vascular with necrosis and degeneration in hepatic cells in addition to reduction in glycoprotein granules. While, the histological changes in kidney tissue included congestion vascular and degeneration in distal and proximal tubules. No obvious histological changes were noticed in liver and kidney tissues in T1 group. The results of biochemical tests for kidney and liver enzymes AST (Aspartate Aminotransferase), ALT (Alanine Aminotransferase) were shown no significant differences in means of the specific activity of these enzymes , AST and ALT in T1 and T2 groups .In contrast T3 group showed significant decreasing in specific activity for both enzymes AST and ALT (14.000 , 12.333) IU/ L respectively . ALP (Alkaline Phosphatase) enzyme was increased significantly in T2 and T3 groups (23.000, 18.000) IU/L respectively compared with T1 and control groups . The results of Creatinine concentration in kidney tissue revealed no significant differences in all groups. In regard to Urea concentration in kidney tissue was decreased significantly only in T3 group (22.666) mg / L compared with the rest of groups.