دراسة مقارنة بين تقييم الخلايا النطفية لعينات السائل المنوي الطازجة مجهريا و جزيئيا باستخدام أختبار المذنب المتعادل (Natural Comet Assay) في الكباش العواسية

Abstract

This study was conducted at College of Agriculture / AL-Qasim Green University for the period from 1/9/2014 and up to 28/5/2015 on Al-awassi rams trained to semen collection artificial vagina but that seven of them did not show a response to that, and that showed in response is only three , These rams were fed on fodder center in addition to the free grazing. The aim of the present study was to study a comparison between sperm cells assessment from the fresh semen samples microscopically and Molecularly by using Neutral comet assay of Awassi Rams. And used in this study Thirty ejections has been evaluated microscopically and ten of these ejections were used in part genetic and evaluated molecular samples compared to samples evaluated microscopically resident molecule through the evaluation of DNA damage of sperm cells and the results of the current study showed the following:. The results of the current study, based on measurements of the ( rate of comet tail length) and (the rate of DNA% in tail) and ( the rate of DNA moment ) significant effect on the level of (P ≤ 0.05) in damage to DNA sperm cells rate for fresh semen samples in Al-awassi rams. There is a positive direct correlation between microscopically semen assessment and molecularly using (Natural Comet Assay.) We conclude from this study that the microscopic assessment gives an initial idea of the level of DNA damage a quotient in sperm cells to fresh semen samples.