The Mutagenicity of The Fungicides Topsin-M and Rizolex in Conidia of The Fungus Aspergillus amstelodami

Abstract

AbstractThe study was aimed to investigating the ability of tow fungicides Topsin-M and Rizolex to induce mutationsresistant to the toxic base analogue 8-aza adenine (aza) on conidia of the fungus Aspergillus amstelodami .Threesublethal concentrations from each Rizolex (0.1, 0.3, 0.5µg/ml) and Topsin-M (0.07, 0.1, 0.14µg/ml) were testedfor their ability to induce the AZA mutants. All these concentrations were sublethal. Two processing were usedto test the mutagenabilty , the first was pretreatment, where in its were treated the conidia with the fungicide fora fixed time before plating on selective media containing agent (8-azaadenine) ; and plate incorporation wherethe fungicide and the selective agent were incorporated together in the growth plate .In both processing, thecolonies were growing on the selective medium considered resistant and their frequency was compared with thespontaneous frequency and with the frequency induced by the positive mutagen UVC. In all experiments thefrequency of the fungicide-induced resistant mutants did not differ significantly from their spontaneous ones