Molecular identification of some causes of ringworm isolated from cattle in Al-Diwaniya region

Abstract

The ringworm is fungal disease results in high economic losses particularly in the fields that used for leather production due to the down grading of healthy skin; a decrease in meat and milk production. this disease caused by several fungi genera like microsporium, epidermophytone, and trichophyton. Trichophyton spp is one of fungi most common zoophilic and dermatophyte fungi that parasitizes and keratinized tissues in human and animal.Our study results showed that percentage of total isolated fungi from infected cattle are (34.2%) by using classical media culture, while it was (100%) using molecular methods (PCR ). in this study isolated two type of fungi are Trichophyton verrucosum and Trichophyton rubrum.Percentage of Trichophyton rubrum that diagnosed in cattle in our study are (33.3%); while was (66.6%) in Trichophyton verrucusum. phylogenetic analysis tree of Trichophyton spp was done by using a primer designed for specific rRNA gene for detection of Trichophyton rubrum and Trichophyton verrucusum.whenever ,Trichophyton rubrum showed high likely similarity with Trichophyton raubitschekii (99%), Trichophyton kanei (99%) and Trichophyton megninii (98%).while Trichophyton verrucusum show similarity with Trichophyton megninii (93%), Trichophyton circonvolutum (92%) and finally similarity with Trichophyton interdigital (93%).Percentage of similarity of the isolates of this study with phylogenic tree analysis is (100%) by using rRNA gene.