GENETIC STUDY OF RESISTANT PSEUDOMONAS AERUGINOSA ISOLATED FROM DIFFERENT CLINICAL SOURCES TO IMIPENEM

Abstract

The antibiotic resistance is now a major clinical problem all over the world . In thisstudy eighty swabs were collected from two different clinical sources; forty samplesfrom wound infections and forty from otitis infections. Out of (23) Pseudomonasaeruginosa isolates there were (14) isolates from wound infections and (9) isolates fromotitis infections. There were (5) isolates resistant to imipenem. The plasmid DNA werestudied to these five isolates. The primary step were DNA extraction to showing DNAplasmid. The secondary step was curing DNA plasmid with acridin orange by usingdifferent concentrations. The results showed that the isolates lost DNA plasmid in512μg/ml of acridin orange and disappear resistance to the imipenem. The genes thatencoded to the resistance Pseudomonas aeruginosa to imipenem may be located onplasmid .These character may led to distribution of resistance to other bacteria bybacterial conjucation.