EXTRACTIONS AND IDENTIFICATION OF SOME ACTIVE COMPONENTS OF IRAQI’S CAPPARIS SPINOSA AND STUDY OF ITS ANTIBACTERIAL ACTIVITIES.

Abstract

Abstract: A Chemical and Biochemical studies have been implemented to different parts of the Plant: capparis spinosa, which is one member of the Capparidaceae, during Flowering and Fruiting Stages. The maximum extraction rate was obtained by water followed by acetic acid and alcohol. The Chemical Tests were done on all the extractions for comparison led to the presence of important types compounds including; Tannin, Fluba-tannins, Saponins, Flavonoides, Terpenoids, Phenols, Resins, Carbohydrates, Amino acids, Alkaloids and Glycosides. Our study revealed that the plant was found to have the highest rate of Potassium in all parts of it through flowering and fruiting periods comparing with Sodium and Calcium.Active materials were isolated from the aqueous extractor using Gel filtration technique with four liquid mobile phases (D.W, Tris-HCl PH=6, Tris-HCl PH=7, Tris-HCl PH=8) through column packed with Sephadex gel G-25.The effluents from the Column were studied Spectrophotometrically in the range of (200-800) nm simultaneously with the general extractor for comparison.The results revealed that the amounts of Tannins increased on fruiting period more than the Flowering period for all parts (Root, Leafs, Flowers) of the Plant except the Fruits where its quantity decrease in the Fruiting period in comparison with the Flowering period.The results clarified that the Moisture increases at Fruiting period more than the flowering period. On contrary, the Percentage of Ash decreases at fruiting period to a level less than the Flowering period for all parts of the plant too.Using Agar-Well Diffusion method, It was found that the aqueous extractor at Flowering period gave the largest inhibition diameter against golden grapes of S.aureus. On other hand, Acetic Acid extractor during flowering stage has offered the higher activity against Escherichia Coli.