CELLULASES PRODUCTION FROM LOCAL ASPERGILLUS SP .. ISOLATE AND ASSESING SOME OF THEIR PROPERTIES AND APPLiCATION 1- ISOLATION OF SOME CELLULASES PRODUCTION FUNGI

Abstract

Twenty three cellulolytic isolates of fungi were isolated f'rum different local SOUI"ces include soil from different locations, maize cobs, wheat straw, wheat bran and some fruits, by using Czapek-Dox media with cellulose as a carbon source instead of glucose at 30 and 45°C. .. spore suspension of each isolate was prepared then it was filtered through a filtration unit (with cotton) and then by filter paper What man 1�.1, the spore count was calculated by hacmocytomctcr and it was insure of their purity. The isolations were identified according to references. The isolates were screened for their cellulolytic efficiency, fin isolates were choosed (A, D, A9, AI~' A1s) which were screened to choose one efficient isolate. It was found that the isolate (Alg) was the highest cellulases producer, it was identified as Aspergillus ,ljJ." Cellulose as a carbon source in the production media, was the selective pressure which we used to induce ccllulases, The aim of this research is to isolate cellulolytic molds and choose one efficient isolate to study the properties of their ccllulascs and the purification of them in further- studies. Using of natural cellulosic sources was a basic step to isolate and select the cellulascs production microorganisms successfully.