Detection of Biofilm in Staphylococci isolated from different clinical infections

Abstract

This study aimed to detection of biofilm in multi drug resistant especially to beta –lactam of Staphylococci spp. isolated from different clinical infections ,which includes (burn swab, wound swab, ear swab, urine samples and pharynx swab ) , We obtained 12 isolates of staphylococci spp . six were to be S. aureus , while other six isolates were to be S .epidermidis characterised by laboratory of bacteriology in teaching baquba hospital and the characterised is certain by using vitek 2 compact system .Sensitivity of isolates against 6 antibiotics were performed by using Kerby-Bauer ,The isolates were appeared different resistant to antibiotics , the percentage of resistance of S. aureus isolates to Erythromycin ,clindamycin , Vancomycin , Gentamicin, Chloramphenicol and Ciprofloxacin were to be(66.7% , 50%, 33.3%, 66.7%, 50%, 33.3%)While the resistance to S.epidermidis to same antibiotics were to be (66.7%, 50%, 33.3%,33.3% 50%, 50%)Detection to Biofilm tests of Staphylococci spp. also performed , detect was performed by two methods micro titer plate and congo red agar , the results appeared that 4 isolates of S.aureus (66.7%) were positive results gave black growth and crystal density while 2 isolates (33.3%) were negative , while S.epidermidis isolates 5 isolates were positive and only one isolates was negative .while the microtiter plates 4 isolates of S.aureus were strong producer and 2 isolates were to be medium ,and S.epidermidis 3 isolates were to be strong producer and 3 other were to be medium.