Effect of alcoholic extract of rosmarinus against some type of entrobacteriaceae

Abstract

Background : In this study the alcoholic extract of rosemary leaves was used to know their inhibition on the growth of some pathogenic bacteria : Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Klebsiella pneumonia and Proteus mirabilis in Muller agar using different concentrations of this alcoholic extract( 200 mg/ml, 100 mg/ml, 50 mg/ml, 25 mg/ml). The mean of inhibition zone were measured, and the most effective concentration was on the following bacteria Salmonella typhi , E. coli, P. aeruginosa, P. mirablis and K. pneumonia respectively, and when ever the concentration of extract was increasing , the mean of inhibition was increased . It was concluded from the results of this study and other similar studies that the natural herbs were able to control the pathogenic microorganism which were resistant to antibiotic, in addition to the important role of these herbs to develop an effective drugs which are safe and cheap .The aimThe main aim of this research was to find out the inhibitory effect of the alcoholic extract of this plant on the following microorganisms:Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Klebsiella pneumonia and Proteus mirabilis. MethodsThe strains which are used in this study have been cultured on Mueller Hinton agar then alcoholic extracts of Rosmarinus at concentration 200 mg/ml were added to the agar then the inhibitory effects were considered by measuring the inhibition zone. ResultsThis results shows that inhibitory effect of alcoholic extract was more effective on Salmonella typhimurium then E.coli. P.aeruginosa, P.mirabilis and K.pneumonia respectively. High effect of the alcoholic extracts of plant Rosmarinus on the different type of bacteria investigation of presence of active compounds (Alkaloids, Glycoside, Tannins, Saponine, Resine and Flavonoids) in this plant were carried out.