ISOLATION OF SOME ACTIVE MATERIALS FROMTrigonella foenum – graecum SEEDS AND STUDY THEIR BIOLOGICALACTIVITY

Abstract

ABSTRACT:This study includes isolation of some materials from Trigoella foenum-graecum seedsand estimation of their percentage like Saponins , Volatils Oils , Glycosides , Alkaloids and proteins .The antibacterial activity study was performed for the active materials which isolated from Trigoellafoenum-graecum seeds against four genus of pathogenic bacteria by using Agar–well diffusion method. It appeared from this study that all of the isolated active materials have inhibitory effect on thebacteria were used . The study also includes separation of free fatty acids from Oils of Trigonellafoenum-graecum seeds estimation of fatty acids percentage by using gas chromatography techniques .Amino acids were separated from Trigoella foenum-graecum seeds by Thin Layer Chromatography( T.L.C. ) . The study also included the determination of metals in Trigoella foenum-graecum seeds, byusing the Atomic Absorption techniques as Mg , Fe , Zn , Co , Al , Cu , Se , Ni and Mn , While thedetermination of the metals which found in considerable amounts by using Flame Photometertechnique like Ca , Na , Li and Ba