Test the inhibition activity of two local Streptomyces isolates by used sawdust as a carbone source

Abstract

Two local Streptomyces (Streptomyces O3 & Streptomyces TS36 ) was usedin this study. The isolate Str. O3 has been isolated from one of Ramadi city soil,the isolate has been selected based on it's ability of producing cellulase throughit's ability of digesting sawdust as culture medium to test inhibition activityagainst some pathogenic bacteria. The isolate Stre.TS36 prediagnosed in collegeof science-University of Al-Anbar has also been used. The isolate Str.O3 wasdescribed phenotypically and physiologically by many cultures media, also hasbeen diagnosed by different temperatures, and it's ability of growth in differentsalt concentrations and different pH was also tested, in which selected optimumconditions to producing inhibition activity against tested pathogenic bacteriaStaphylococcus aureus& E. coli..Result showed the ability of the two selected streptomyces used in studying it'sutilization of sawdust as a carbon source for growth and production of inhibitionactivity against pathogenic bacteria, in which the solid culture and liquid culturemethods were used to grow the two local isolates to produce the inhibitionactivity in presence of sawdust, both isolates were able to produce the inhibitionactivity in both solid and liquid cultures, the isolates Str.O3 and Str.TS36 wereable to produce inhibition activity in test cultures 2,3 and 4 containing 0.3, 0.7and 1.5 g/ml of sawdust respectively against Staphylococcus aureus similar to orlittle better than control culture that does not contain sawdust, also the isolatesStr.O3 was able of producing inhibition activity in the same previous test culturesagainst E. coli, the isolate Str. O3 showed high stability in high temperature inwhich it was able to grow and produce inhibition activity in temperature 40C0comparing with isolate Str.TS36 that was able to grow and produce inhibitionactivity in 28 C0.