The Phenolic Contentand Antibacterial activity of Cladium Mariscus Seeds Extract

Abstract

AbstractCladium mariscus is one of the plants belonging Cyperaceae family. Itis a common plant in ALHammarmarshes, eastern - south of Iraq.Polyphenols constitute one of the largest and widelydistributed groups of natural products in the plant kingdom. So the aim of the present study wasmeasurement of total phenolic content in the poly phenolic extract of the plant seeds, by using Folin–Ciocalteau reagent, and study the antibacterial activity of the poly phenolic extract. Cladiummariscus seeds were extracted in methanol: water (70:30 v/v). The optimum conditions forwavelength, and standard substance were 550 nm, and Gallic acid, respectively.Theantibacterialactivity of methanol: water extract was examined against two Gram positive bacteria( S. aureus andS. coccus) andtwo Gram negative bacteria (E.coli and P. aeruginosa) pathogenic bacteria strains, byusing the well disk diffusion method, showed that the methanol: water extract had a clear inhibitioneffect against microbes at ( 50%, 75% and 100% w/v) concentrations.