Diagnosis and study of the effectiveness of the enzymes invertase and cellulase in some males pollens of date palm Phoenix dactylifera L

Abstract

This study was conducted to assess the effectiveness of the two enzymes invertase and cellulase in the pollen grains of some males cultivars of date palm and their effects on the percentage of pollen viability. Results showed that the pollen of Ghannami akhder had the highest rate of effectiveness of the enzymatic of invertase and cellulose( 128.52 , 144.51) unit / ml respectively , While the lower effectiveness recorded in Khkri ahdi (75.51 and 118.14) units / ml respectively. There is a significant effect of freezing storage of pollen Factor in the of enzymatic effectiveness of invertase and cellulose. As for the interaction between the cultivar and the treatment of storage it has surpassed the treatment of Ghannami akhder pollen before storage Compared to other treatments in the enzymatic effectiveness of invertase and cellulose (136.90 and 152.81) units / ml, respectively. All the male cultivars studied was of vital rates ranged from (89.38%) in Khkri ahdi to (96.75%) in the Ghannami akhder, did not significant effect of freezing storage factor on the vitality pollen varieties studied. The higher value of the enzymatic effectiveness of the invertase and cellulose at a temperature of 30 cº (30.63 and 33.10) unit / ml, respectively. As for the interaction between the cultivar and the incubation temperature, the treatment of Ghannami akhder and incubation temperature of 30 Cº, had significant rate(35.30 and 37.50 unit / ml for invertase and cellulose) compared with the treatment of Khkri ahdi and incubation temperature of 35 Cº which recorded the lowest rates (17.50 and 22.70) unit / ml, respectively.