تأثير التعدد الشكلي للنيوكليوتيدات المفردة في جين اللاكتوفيرين في بعض الصفات إلانتاجية والتناسلية لسلالات الماعز في العراق

Abstract

This study was conducted at the research station of the Agricultural Research Department of the Ministry of Agriculture located in Abu Ghraib (20 km) west of Baghdad. During the period from Mid-August 2016 to Mid-October 2017. The intention limitation study the polymorphism of Lactoferrin gene. The relationship to different goat breeds and their relationship and their relationship to some productive and reproductive characteristics of a group of 65 female goats were extracted from a sample of blood (5 ml / goat), DNA was extracted for all samples. Using the frontal initiator of each double gene, with a concentration of 100 piccolos / μl, Results after polymerase chain reaction (PCR) and volume (20) micro liter to the company Macrogen Humanizing Genomics in South Korea - Seoul to determine nucleotide sequencing sequences with Sequencing DNA technology. The distribution of genotypes ratios at the site of the lactoferrin gene in the sample of different goat breeds was reached for the local goat line 96.97, 3.03 and 00.00 % of the genotype of TT, TC and CC sequentially. In the Cypriot goat breed, they were 100.0, 0.00 and 0.00 % for the TT, TC and CC sequentially, and the crossbred goat It has reached 86.67, 6.67 and 6.67 % The TT, TC and CC genes were sequentially, result in decanted highly significant (P≤0.01) , Has observed effect of genotype of the gene in weight description at birth with significant differences (P≤0.05) no effect was observed for weight in weaning , total increase rate and period between two births and some milk components (milk density and degree of dehydration). All other studied traits were significantly affected (P≤0.05) for daily milk production and non-fat solids . The results of the milk components (fat, protein and lactose) Showed highly significant differences (P≤0.01) , The allele frequency was for the two alleles T>C the resulting from the analysis of lactoferrin gene Non-symmetric (0.97 and 0.03) each everyone .We can conclude from this study the polymorphism of Lactoferrin gene that it can be used to develop genetic improvement in goat breeds, The economic a plus of the breeding projects through the selection of crossbreeding Distinguished individuals and Contains Genotype that achieved the best performance in the productive flock