Frequency of genotypes of MTHFR gene for people with Congenital heart defects in the province of Thi Qar

Abstract

Abstract:The present study aimed to detect the role of MTHFR gene and their relationship in the incidence ofCongenital heart defects in Dhi Qar province, through the use of PCR- RFLP technique on this basiswere collected 120 blood samples (from Nasiriyah heart Center) in tubes container on EDTA frompatients with congenital heart defects ages ranging between (a month - 17 years) and 110 samplesfrom healthy people ages ranging between (a month - 17 years) and saved directly at a temperatureof - 20 ° C for use in the extraction DNA and the PCR technique.The results of the statistical analysis of the lack of correlation between polymorphism of the geneMTHFR and the incidence of congenital heart defects when compared to the comparison group Andeach of the mutant homogenized style(CT) (OR= 0.86 ; 95%CI= 0.41-1.81) The style of the mutantdifferential(TT) (OR=0.42 ; 95%CI= 0.22 - 0.79) .The results of the current study has shown an increased risk of congenital heart defects for womenwho have the mutant heterozygous (CT) almost twice (OR= 1.63 ; 95%CI=0.61-4.29). and theresults also showed that who have the mutant heterozygous (CT) and within the age group (month - 9years) has increased their risk of congenital heart defects (OR = 1.5 ; 95%CI= 0.36-6.23), resultsshowed an increased risk of infection with parents smokers about almost three times (OR = 2.5) andthe period of confidence (0.76 - 8.22) the presence of mutant homozygous (TT) while that possessthe mutant heterozygous (CT) increased their risk more than injury twice (OR = 2.13) and the periodof confidence (0.71 - 6.40).