Phenotype and Genotype of Virulence Factors in Escherichia Coli Isolated from Different Clinical Samples

Abstract

Escherichia coli is the principal causative agent of most infection of bacterial origin, and extraintestinal pathogenic E. coli (ExPEC) is responsible for 80% of infections of urinary tract in human bieng. The aim of this work was to study the distribution of some virulence factors of Escherichia coli in different type of human samples. This study was carried out in Baghdad city-Iraq from January to June 2019, included 70 clinical samples (Blood, Urine and Wound samples) with positive culture for E. coli. Each sample was managed bacteriologically to isolate causative bacteria as mentioned in standard systems of bacteriology Lab. The DNA was extracted from all E. coli isolates for detection of fim H and Kpc gens (important virulence factors of bacteria) by PCR and for determination resistance antibiotics these isolates. The present study showed that the highest rate of E coli isolates (78.57%) was from urine samples of patients with UTI and the lowest percentage was from blood samples (8.57%). The study show highest percentage of E coli isolates (95.43%) was positive for adhesion factors, followed by 91.43% was positive for biofilm formation, 70% was capsule producer, while 21.43% of E coli isolates was multidrug resistance (MDL) (p: < 0.05). The study revealed that 95.71% of E coli isolates was resistant for cefotaxime, 94.29% was resistant to aztroneom while 2.86% was resistant to amikacin. The study revealed that 95.71% of E coli isolates was with adhesion factors and 70% of E coli isolates capsule producer as phenotypic virulence factor while 95.71% of E coli isolates was positive for Fim H gene and 4.29% was positive for Kpc gene (as genotype virulence factors).Conclusions: nearly quarter of E coli isolated was multidrug resistant and fim H virulence gene was the most gene detected in those isolates