PREPARATION AND EVALUATION OF LOCAL MEDIUM FOR CULTURE AND GROWTH OF Trichomonas vaginalis ISOLATED FROM PATIENTS IN MOSUL, IRAQ

Abstract

ABSTRACTThis study was conducted to prepare a local culture medium from orangeleaves for cultivation of Trichomonas vaginalis. Five culture media, namely:Oxide Trichomonas media (CM 161), CPLM, Barbarowski, Protose pepton andPavlova, which are used for clinical diagnosis of vaginal trichomoniasis werecompared in vitro to this new medium. Growth studies using fresh clinicalisolates from different patients showed clear ability of Trichomonas to grow onthis medium. The organisms inoculated initially (25´l04 cm-3) into this mediareached a population of (78´l04 cm3) organism in 72 hours. Although (CM 161)and (CPLM) media allows more prolific growth over a shorter period of time(24 hours). It was obvious that the orange leaves media (OLM) was suitable fordetecting T. vaginalis in patients with vaginitis.