Preparation Bradyrhizobium japonicum Inoculum's tolerance the salinity and develop suitable carrier material

Abstract

Biofertilizer play avital role offsetting the deficit produced by nutrients loss and has great contribution in supporting the continuous agriculture effectively. thus an attempt to isolate rhizobium bacteria from soybean plant roots from different field in Baghdad and Anbar governorates was carried out and identified as Bradyrhizobium japonicum.These isolates were tasted for salinity tolerance, ability to dissolve phosphate compounds, producing Indol acetic acid , producing sidrophore, thin 5 different local materials as a carriers were added to the selected isolates, Glucose sugar was also added to support the selected material, Inoculation efficiency for nitrogen fixation and salinity tolerance were carried out after 30 days of storage period at different temperature .Results showed the following :15 isolates belong to B. japonicum were obtained .Isolates Kd2, Kr3, Kr2 showed there intensive ability of growth (+++) at salt concentration of NaCl (3-5)% with change in color of colonies to yellowish brawn.Isola Kd2 showed significantly in superiority in dissolving phosphate compounds rate diameter of 11.6 mm on solid media.and able to produced indole acetic acid (IAA) in media by amount of 13.60 and 11.5 mg /L before and after salinity adaptation .Isolates Kd2A and Kr2A showed ability in resisting toxic effect of Dithen and Kitolite materials after salinity adaptation with 0.1% concentration of salt. Powdered dates nuclear showed highest rate in loading isolates cell Kd2A isola with density 7.66 Log cfu/ml the process of 5% glucose support resulted in increase in numerical density rate 8.86 Log cfu/ml with using 3 ml volume of inocula par 5 gm carrier.4 C storage temperature for 30 days period showed the possibility of isola cell remaining active and reproducing them back with density of 6.96 Logcfu/ml. the isola kept its ability to grow at salinity concentration of rate 4.82 ds/m and nitrogen fixation at rate 2.71 and 2.49 mg N/L under salinity level of 3.82 and 4.82 ds/m.