Effect of different levels of pesticides and in growth and numbers of Azotobacter Bacteria cells under period of incubation.

Abstract

Two laboratory experiments were carried out with (CRD) design. Each experimentswere included (144 ) experimental units for each incubation period and comes of (12 )bacterial isolation , ( 4) level of herbicide mixture ( 0.0 , 75+25 , 150+50 , 300+100 ) and(3 ) replication five period of incubation ( 3, 7, 10 ,14 , 21 ) days were used for measuring (MIC ) of growth and bacterial cells number .The results showed that the isolation ( I1,I2 ,I3 )which were taken from treatments of mixed pesticide considered as resistant local isolationsto mixed pesticide compared with the isolations ( I4, I5 , I6 ) which were taken from untreatedtreatment with mixed pesticide .So the ( MIC ) of ( I1 ,I2 and I3 ) isolation is ( C4 ) for (3 )days incubation and growth in all concentration for the period of incubation (7 ,10 , 14 and21 ) days , while the isolation ( I4 , I5 and I6 ) was not recorded growth and numbers with (3 )days incubation the ( MIC ) in ( C4 ) for the incubation period ( 7 , 10 , 14 and 21 ) days . Theresults also showed that the isolation ( I7 ,I8 and I9 ) which were taken from treatments ofpesticide mixture addition as considered resistant local isolation for mixture of pesticidecompared with isolation ( I10 , I11 and I12 ) .