Permeability perturbation during ageing of Mungbean Cuttings and its Control by Supplying Parsley Extract II: ios uptake (influx)

Abstract

Abstract : Quantitative determinations of the average uptake for K+, Na+ , Mg+2 and Ca+2 ions has been measured. These parameters was considered as indicator of the influence of aging on permeability perturbation of cytoplasmic membranes in terms of adventitious root formation in aged cuttings of mungbean compared to fresh cuttings . The data revealed the followings. a-A decline in percentage of all the above ions in cutting aged for 3- days in d/H2O , except the surprising increase in % of Na+.b-After treatment of fresh & aged cuttings with optimal concentrations of KCl, NaCl, MgSO4 and CaSO4 for 24h , the % of ion uptake was increased in different parts of cuttings , but the increasing % was differ according to different parts of cutting as well as for different ions . Where as , in cuttings aged in d/H2O , the % of ion uptake was increased significantly after its treatment with the optimal conc.s of the above salts in all parts of cuttings . The % was higher and beyond 100% in some parts of cuttings . c-For controlling aging phenomenon, cuttings were kept in aqueous extract of parsley seeds (1%) instead of d/H2O . This extract completely overcome (stopped) these processes occurring during aging , that lead to diminishing rooting response to subsequent auxin trentment. Thus , cuttings kept in parsley extract were responed to inductive auxin treatment as it was the case in fresh cuttings ,in terms of membrane repain .Thereafter , prevent ions influx that occurs due to permeability perturbation . d-The average of ion influx in the current manscript (2nd part of the study) is more than the average of ion efflux (1st part of the study that has been already published) (Shaheed & Jabor, 2009).