SEROLOGICAL DETECTION OF JOHNE'S DISEASE IN CATTLE USING ELISA TECHNIQUES

Abstract

This study was designed to diagnose Johne's disease infection in serum of cattle. Ninghty two blood samples were taken from cattle of Ninevah province and tested for detection of antibodies against Johne's disease. Indirect ELISA Kit for Mycobacterium avium subspp. Paratuberclosis used to examined animals serum samples, Clinically cattle show emaciation, decrease in milk production and some cases of persistence diarrhea. ELISA test show that 1.08 % of serum samples were positive and all the seropositive animals were more than 2 years aged. In conclusion the cattle were infected by Johne's disease and animals above 2 years were more susceptible to infection.