Appreciation survey of natural biodegradation of crude oil which cause soil contamination and trying to diagnose the bacterial species which cause this biodegradation

Abstract

This survey includes proven analysis of polluted soils of Kirkuk crude oil for three sites in Baiji refineries trying to know the bacterial species which are responsible on biodegradation and it’s quantity on the crude oil. Samples of polluted soils were collected with the control samples for four seasons during 2002 and 2003 and they were analyzed. The survey indicates the following: 1-Adecreasing of pollution ratio was happened by the time where the ratio of the biodegradation during the four seasons starting from winter 2002 to Autumn 2003 was 18.5 % 2-Adifference among the isolated bacterial species and that was according to the temperature degrees which were used in incubation of soil samples. Acinetobacter SPP was the predominant during the four degrees of incubation.3-Flavimonis oryzihabitans makes the biggest biodegradation when the sample was incubating on 20C° where the ratio of decomposition was 68.269%, also the same kind makes the biggest decomposition ratio during incubation in 25C° where the ratio was 56.724% comparing with Acinetobacter SPP .While incubation in 30C°, just Acinetobacter SPP. was decomposed where its ratio was 51.335%. On the other hand, during incubation in 35C° Aeclerica SPP makes the biggest decomposition ratio which was 72.296% compared with the other two kinds